Enzymology (summer semester) KZ-JO-06-01-000006
Isolation of enzymes.
Determination of enzyme kinetics based on absorption measurements.
Determination of Michaelis constant based on the Michaelis–Menten equation.
Detection of substrates and products of enzymatic reaction.
Studies on the activity of peroxidase from cabbage - preparation of peroxidase, the oxidation reaction of guaiacol, determination of the peroxidase activity.
Studies on the activity of phosphatase from potato - preparation of phosphatase, the hydrolysis reaction of p-nitrophenylophosphate, determination of the phosphatase activity.
Studies on the activity of invertase from yeast - preparation of invertase, the hydrolysis reaction of sucrose, determination of the invertase activity.
Supplementary literature
Course coordinators
Term 2023/24-Z: | Term 2022/23-L: | Term 2023/24-L: |
Assessment criteria
The assessment will be based on the student presence and activity during the laboratory class, and the oral tests on the student's knowledge of the topics related to the lab actvity.
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: