Molecular biology 6.15.BTM-BM

Lecture:
- basic concepts used in molecular biology, history of the discovery of a genetic factor
- DNA and RNA types; construction and functions; organization of genetic material in a pro and eukaryotic cell
- changes in the structure of chromatin - chemical modifications of histones in connection with gene activity
- chromosomes, telomeres and telomerase, chromosomal structural aberrations, in situ hybridization, Philadelphia chromosome
- replication and its mechanism in pro- and eukaryotes, mechanism of PCR reaction
- homologous recombination, damaged DNA repair, diseases caused by inheritance of variable size DNA segments and chromosome aberrations
- transcription, post-transcriptional pre-mRNA processing, mechanism of RNA polymerase action, negative and positive transcription regulation, repressors and inducers, types and effects of transcription factors
- protein translation and degradation
- epigenetic

Laboratory:
- isolation of genomic DNA from bacterial cells
- isolation of total RNA from bacterial cells
- detection of mutations in the CCR5 Homo sapiens gene by PCR
- detection of the presence of Salmonella sp. cells in food products by PCR